Biochemistry HEC,UHS, PNC, Curriculum, Course Outline, Syllabus & Lab Practical List

Biochemistry HEC Curriculum 

BScN, BSN, Syllabus,Biochemistry HEC,UHS,PNC Curriculum, BScN 1st Year, Biochemistry Course Outline, Biochemistry Syllabus, Biochemistry Outline, Biochemistry Nursing Curriculum.

Subject Semester Hours 

     In the 136 Credit hour program ,there 3 hours for Biochemistry.With 2 theory and 1 Lab  Credit hours In the "General Courses to be chosen from other departments" Category.

Course Description

    This course is intended to provide the health professional with an understanding of the major organic substances of living organisms, proteins, carbohydrates and lipids, their structure analyses and biochemical functions. It also provide an introduction to the chemistry of bioenergetics, metabolism, biosynthesis and molecular biology 

Course Objectives

    At the end of the course students will be able to: 1. Describe the basics of organic chemistry with emphasis on the biomolecules and reactions encountered in biochemistry of proteins, carbohydrates, lipids, enzymes and hormones. 2. Perform experiment to study the chemical & physical properties of biomolecules. 3. Relate the basic knowledge of biochemistry for better understanding of the physiological functions of the human body.

Bio-Chemistry

Sr.No	Topics
1.	Unit:I:Basic concepts of Chemistry Review the basic concepts of matter and elements mixture, compounds, chemical reactions, equations & bonding. Importance of Chemistry in nursing+Matter+Elements Mixtures & Compound
2.	Structure of Atom (Periodic Table)+Chemical formula+Chemical Reactions
3.	Equations +Bonding +Redox Reaction +Acid Base
4.	Uni:II:Basic concepts of Organic Chemistry Definition of Organic Chemistry & Compounds+Classification of Organic compound
5.	Properties of Organic compound+International Union Nomenclature of Alpha
6.	Compounds (IUPAC)+Hydro Carbons
7.	Alkanes, Alkene & Alkynes +Isomerism
8.	Functional groups +Macromolecule of human body
9.	Uit:III: Metabolism and Adeno-Tri-Phosphatase (ATP) Define metabolism, anabolism, catabolism +Define Andensoin Triphosphalic (ATP) and discuss its relationship with catabolism and anabolism.
10.	Define the following terms, gluconeogenesis, glycogenesis, glycogenolysis, transamination, deamination & ketosis+Briefly discuss the following metabolic pathways for carbohydrate, protein & fats in terms of Glycolysis. Kreb cycle & electron transport chain.
11.	Unit:IV:Chemistry of Carbohydrates Structure of Carbohydrates+Classification of Carbohydrates
12.	Properties of Carbohydrates+Biological significance of Carbohydrates
13.	Unit :V:Chemistry of Lipids Structure of Lipids+Properties of Lipids
14.	Biological significance of Lipids Classifications of Lipids+1. Simple lipids +2.Compound Lipids
15.	Derived Lipids +Fatty Acids
16.	Fats & oil+Saturated and unsaturated fatty acids
17.	Uses of fats in the body+Physical and chemical properties of fats +Distinguish between soap and detergents+Explain briefly the role of phospholipids in cell membranes+Role of steroids (cholesterol )
18.	Unit:VI:Chemistry of Proteins General chemistry of amino acids+Composition +Amphoteric nature +Essential,nonessential  aminoacids
19.	Polar, Non polar and zwitter ion+Dipeptides+Structure of Proteins ,Primary,Secondary,Tertiary,Quaternary
20.	Functions of Proteins+Biological importance of Proteins
21.	Classification of Proteins on the basis of ,Solubility,Composition,Biological functions
22.	Properties of Proteins ,Colloidal nature,Denaturation of Protein
23.	Unit:VII:Nucleic Acid & Nucleotide Review of the cell+Structure of Nucleic acid+Importance of DNA and RNA
24.	Chemistry of Nucleic Acid, Nucleosides and Nucleotides+Structure of DNA & RNA
25.	Functions of Nucleic Acid+Biological importance of Nucleotides
26.	Unit:VIII:Enzymology Importance of enzymes+Enzyme as Protein+Properties of Enzymes +Classification,Significance
27.	Factors affecting enzyme activity+Enzyme Specificity+Mechanism of Enzyme action+Activators and inhibitors (compitative+Non competitive+uncompitative)
28.	Factors affecting Enzymes activity,Concentration,Ph,Temperature,Time
29.	Apoenzymes, coenzymes & co factors+Co-Enzymes & its classification+Enzymes in clinical medicines
30.	Unit:IX:Bio-energetics and Metabolism Definition of Bioenergetics,Biogenetic Metabolism
31.	Define Bioenergetics Biological+Oxidation,Oxidation,Briefly discuss the concept of free energy
32.	Electron transport chain+Explain the role of ATP in Linking Catabolism and anabolism
33.	Electron Carriers+Biological Redox Reaction
34.	Unit X: Metabolism of Carbohydrates Explains the metabolism of CHO,Discuss the importance of glucose in blood and its dependency by various
35.	Discuss the glycogensis and glycogenolysis,Describe the role of oxidative glucose catabolism in the citric acid cycle.
36.	Describe the role of gluconeogenesis,Discuss the overall scheme of carbohydrate metabolism
37.	Unit:XI:Metabolism of Protein Review the digestion and absorption of Proteins
38.	Nitrogen balance and its significance+General pathway of Protein metabolism
39.	De-amination,De carboxilation, transmissionn + formation of urea and ammonia disposal
40.	Unit XI: Metabolism of Fats Describe the mechanism of fatty acid oxidation
41.	Discuss the amount of energy produced during the oxidation of a fat.
42.	Explain the significance of the role of ketone bodies.
43.	Unit:XIII:Hormones (Communication among cells & tissues) Introduction of hormone+Classes/Classification of Hormones,Steroid
44.	Hormone+AminoHormone,Peptide Hormone
45.	Mode of Action of steroid & peptide hormones+ To become familiar with the cardiac, pineal and gastrointestinal hormones.

Topics to be Covered in Laboratory 

1. Review Lab 

2. Properties of Organic compounds 39 

3. Cell TBD (Total Body Density) 

4. Carbohydrates 

5. Lipids 

 6. Proteins 

 7. Metabolism 

 8. Enzyme kinetics 

 9. Lab evaluation 

Lis of Laboratory Practicals

Sr.No	Practical
1.	First aid precautions and working in laboratory
2.	Glass ware use in laboratory
3.	Solutions and their types
4.	Molischs test
5.	Iodine test
6.	Benedict test
7.	Saliva test
8.	Phynylhaydrazine test
9.	Millons test
10.	Ninhydine test
11.	Biuret test
12.	Determined glucose in serum
13.	Determined creatinine in serum

Recommended Books

1:Chatterjea MN. (2004) Textbook of Biochemistry for dental /nursing / pharmacy students 2nd ed, New Delhi : jaypee.